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KMID : 0545120000100040522
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 4 p.522 ~ p.528
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Antiviral Activity of Antibiotic Peptaibols,Chrysospemins B and D,Produced by Apiocrea sp.14T against TMV Infection
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KIM, YOUNG HO
YEO, WOON-HYUNG/Kim, Young Sook/Chae, Soon Yong/Kim, Kap Sik
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Abstract
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A total of about 300 fungal isolates from forest habitats were screened for inhibitors of tobacco mosaic virus (TMV) infection using its local lesion host, Nicotiana tabacum cv. Xanthi nc. One of the isolates, 14T, showed a strong activity against TMV infection, and was identified as an Apiocrea sp. based on its morphological characteristics. Rice was an optimum culture medium for its fermentation, and two antiviral compounds, KGT 141 and KGT 142, were resolved from the rice culture through column chromatography, TLC, and HPLC. By NMR and FAB-MS, the two compounds were identified as chrysospermins B (KGT141) and D (KGT142), both of which are peptaibols with 19-mer amino acids possessing an acetylated N-terminus and a hydroxy-amino acid (tryptophanol) at the C-terminus. Both compounds showed inhibitory activities against TMV infection, but chrysospermin D showed the stronger activity than chrysospermin B. The former exhibited 96.3% of TMV-inhibitory efficacy at the concentration of 100㎍/㎖ and 54.7% at 10㎍/㎖, respectively. Furthermore, the chrysospermins were highly cytotoxic toward cancer cell lines of PC-3 (prostrate) and K562 (leukemia), and inhibited growth of the Gram-positive bacteria tested, especially the plant pathogenic bacterium Corvnebacterium lilium. To the best of our knowledge, this is the first report on the inhibition of plant virus infection by antimicrobial peptaibols.
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